Development of specific experimental systems for flavinogenesis using non-growing cell of Eremothecium ashbyii.

Studies on the biosynthesis of riboflavin; nitrogen metabolism and flavinogenesis in Eremothecium ashbyii.

The effects of certain purines and pyrimidines upon the production of riboflavin by Eremothecium ashbyii.

Eremothecium ashbyii is one of a number of organisms which produce considerable amounts of riboflavin, most of which is secreted by the cells into the medium (Guilliermond, 1935). The investigations of Schopfer (1944) and Schopfer and Guilloud (1945) resulted in the development of a synthetic medium for this organism which permitted growth and riboflavin production to occur under chemically def...

Some nutritional requirements for biosynthesis of riboflavin by Eremothecium ashbyii.

Eremothecium ashbyii Guilliermond is a remarkable organism because of its capability of synthesizing relatively large amounts of riboflavin under appropriate conditions. This vitamin is synthesized to a limited extent by a rather wide variety of microorganisms (Pridham, 1952), but those known particularly for their riboflavin producing abilities along with E. a&hbyii are Ashbya gosypi'i (Wicker...

The Synthesis of Cellulose by Enzyme Systems from Higher Plants.

In 1957 Glaser (1) obtained a particulate fraction from Acetobatter xylinum which catalyzed the incorporation of the glucosyl moiety of uridine diphosphate n-glucose into cellulose. It seemed probable that this most important structural polysaccharide of higher plants was synthesized by a similar mechanism, but attempts to bring about its formation from uridine diphosphate u-glucose by enzyme s...

The utilization of the ureide carbon of adenine in the biosynthesis of riboflavin in Eremothecium ashbyii.

There is considerable evidence that a critical reaction in purine synthesis is the closure of the purine ring by the addition of a “formate” group to 4-amino-5-imidazolecarboxamide ribonucleotide (1). Apparently the “formate” is added as an active intermediate, since formate as such has little effect in stimulating purine synthesis. Indeed, there is a demonstrable difference in the utilization ...